Diagnostyka cytologiczna – wytyczne do właściwego pobierania materiału/Cytologic diagnosis – guidelines to a proper preparation
Key points
Smears need to be dried at room temperature, prior to staining and/or shipping. If samples are packed while still moist, cells will suffer a wide variety of artefacts, often resulting in destruction of cells and non-diagnostic results.
Staining the specimen
A variety of Romanowsky-type stains are available that are well suited for cytologic specimens. (e.g. Diff-Quick®, Hema-Quick®). The staining procedure will usually follow the manufacturer's instructions, but a few adaptations can be helpful. Dense, highly cellular smears may need longer staining time, also weakened staining after long use of the solutions can sometimes be compensated by longer, or more frequent dipping.
Good quality of staining is prerequisite for good diagnostics! If the microscopic evaluation shows that colours are too pale, the staining procedure can simply be repeated.
A convenient way for maintenance of staining solutions is to use aliquots and store them in airtight containers (e.g. urine sampling beakers, plastic cups with snap on lid).
However, if staining solutions are contaminated with bacteria they need to be replaced, contamination happens easily, especially with frequent use or higher ambient temperatures.
If samples are sent away for diagnosis, at least one of the slides should be sent unstained, to allow the pathologist to [...]
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