Diagnostyka cytologiczna – wytyczne do właściwego pobierania materiału/Cytologic diagnosis – guidelines to a proper preparation
In highly vascular tissue, or if good exfoliation of cells is expected (e.g. in lymphnodes) FNB (fine needle biopsy) is the preferred method of sampling.
In contrast to FNAB technique FNB is performed by placing a needle without syringe into the lesion. While inserting and retracting the needle in different directions it is rotated in order to punch out enough cells.
The further preparation is performed like described for FNAB. If more than one lesion is present, each of them has to be sampled. In this case special care has to be taken: correct slide labelling is necessary and localisation and sampling sites/labelling should also correlate with the anamnesis. Masses should be aspirated in more than one location, whenever size allows. Rapidly growing neoplasia sometimes yields only necrotic debris centrally, which is not diagnostic. On the other hand, inflammatory lesions can be surrounded by highly active mesenchymal proliferation that can even mimic sarcoma.
In generalised lymphadenopathy, mandibular nodes should not be chosen as sole location for biopsy. Frequently there will be inflammatory changes in nose or throat, contributing reactive changes to the lymph node populations that can make diagnosis more complicated. If possible, other lymph nodes should be sampled preferentially (please note down the locations).
Liquid samples
If aspiration yields fluid, the sample should be [...]
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