Diagnostyka cytologiczna – wytyczne do właściwego pobierania materiału/Cytologic diagnosis – guidelines to a proper preparation

Slide preparation

Touch imprints are best made before and after cleaning of a superficial lesion (slide labelling!). A slide is touched softly onto the ulcerated skin. Care must be taken not to wipe over the lesion, because cells are easily destroyed in that way and there are only chromatin strands visible on the cytologic preparation.

If big crusts are present, their lower surface should also be imprinted, because some microorganisms e.g. Derma­tophilus congolensis are detected frequently in this location.

Scrapings

Are performed predominantly in order to detect ectoparasites, sometimes fungal hyphae or spores can be found, too. The affected part of the skin is clipped, if necessary, preferably without removing scales or crusts. A scalpel blade is prepared with few drops of paraffin oil. With this instrument the skin is scraped several times, depending on the desired depth of the scraping.

The sampled material is applied to a glass slide, maybe with another drop of liquid paraffin, a coverslip is applied. This preparation is scanned unstained in the microscope at 4x or 10x magnification.

Indirect smears

Are made by gently rolling a swab or a cytobrush over the surface of the lesion and afterwards transferring the gathered material onto a glass slide, [...]

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